In vitro and in vivo investigations of mild hyperthermic modulation of palladium-bacteriopheophorbide (WST09)-mediated photodynamic therapy doses.

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Quantitative studies were performed to determine how mild hyperthermia (HT, 37--42°C) modulates photodynamic therapy (PDT) using a sub-cellular targeting photosensitizer aluminum phthalocyanine tetrasuflonate (AlPcS 4) and a vascular-targeting photosensitizer Pd-bacteriopheophorbide (WST09). The ultimate goal is to improve WST09-mediated PDT treatments of prostate cancer, which are currently under investigation at the University Health Network. Simultaneous HT enhanced WST09-mediated PDT of rat prostate endothelial cells in vitro (HER of 1.42--1.67 at 41--42°C). Using the PDT threshold dose as the in vivo metric, the first quantitative relationship between temperature and PDT threshold dose was determined. HT (40--42°C) reduced the PDT threshold dose by 48 +/- 10% and 27 +/- 9% for WST09 and AlPcS4 respectively. Finally, by coupling the in vivo data with a mathematical model of optical/thermal propagation in tissues, it was shown that adjuvant HT may be an effective strategy to modulate PDT treatment volumes or homogeneity of response.

The Physical Object
Pagination143 leaves.
ID Numbers
Open LibraryOL20239001M
ISBN 100494025158